Want to learn more about the Cellular Thermal Shift Assay?

The following references give the fundamental information and relevant background to the different formats of the method.


The pioneering CETSA publication from 2013:

Monitoring Drug Target Engagement in Cells and Tissues Using the Cellular Thermal Shift Assay

Daniel Martinez Molina, Rozbeh Jafari, Marina Ignatushchenko, Takahiro Seki, Andreas Larsson, Chen Dan, Lekshmy Sreekumar, Yihai Cao, Pär Nordlund            Science 05 Jul 2013: Volume 341, Issue 6141


The first published use of mass-spectrometric readout:

Tracking cancer drugs in living cells by thermal profiling of the proteome

Mikhail M. Savitski, Friedrich B. M. Reinhard1, Holger Franken, Thilo Werner, Maria Fälth Savitski, Dirk Eberhard, Daniel Martinez Molina, Rozbeh Jafari, Rebecca Bakszt Dovega, Susan Klaeger, Bernhard Kuster, Pär Nordlund, Marcus Bantscheff, Gerard Drewes
Science 03 October 2014: Volume 346, Issue 6205


The first publication showing that CETSA can be used to screen a library of compounds for target engagement:

CETSA screening identifies known and novel thymidylate synthase inhibitors and slow intracellular activation of 5-fluorouracil

Helena Almqvist, Hanna Axelsson, Rozbeh Jafari, Chen Dan, André Mateus, Martin Haraldsson, Andreas Larsson, Daniel Martinez Molina, Per Artursson, Thomas Lundbäck & Pär Nordlund
Nature Communications 24 March 2016: Volume 7, Article number: 11040