Bridge findings from cell systems to tissues and whole blood
Translational studies assess whether relationships observed in cellular models are maintained in relevant tissues and more complex biological systems. This phase focuses on linking exposure to pharmacodynamic effect, determining which matrices and time points are informative, and evaluating whether assay endpoints remain practical and interpretable across systems. The goal is to establish evidence that supports dose selection, sampling schedules, and endpoint choices for subsequent pharmacology and safety studies.
Study design decisions center on selecting tissues and matrices that best reflect the indication, such as whole blood, specific organs, or surrogate matrices. Sampling strategies are defined to capture relevant dose levels and time points, while analytical methods are selected to relate exposure to biological effects. Attention is given to sources of variability, including donor differences, tissue handling, and matrix interference, to ensure that observed effects are robust and reproducible.
What you see in cells, you are likely to see in patients
In translational studies, CETSA can be applied to assess target engagement directly in tissues or complex biological systems. This helps determine whether exposure–response relationships observed in cells extend to more physiologically relevant contexts and supports decisions on which endpoints are ready to progress into in vivo studies or biomarker verification.
Translational studies is enabled by our Unbiased- or Targeted Profiling Services
Proteome Wide Profiling
Broader investigation of compound effects across the detectable proteome.
Targeted Profiling
Validate mechanistic hypotheses and confirm that on-target engagement occurs in the intended cellular context.
Webinar: How CETSA® Enhances Translational Studies – A Sitryx Case Study
In this on-demand webinar, Jon Ellery, Director of Biology at Sitryx, shares how CETSA technology helped Sitryx assess target engagement and improve translational confidence in small molecule drug discovery.
Is your compound actually therapeutic?
Learn how CETSA confirms target engagement across cell lines, animal models, and human primary cells—providing physiologically relevant insights that streamline drug discovery.
What is CETSA?
Why work directly in cells?
Are you using the right tools?
Why early target engagement matters
Let’s talk
For us, every project is a partnership.
Send us a message or book a free scoping call with one of our experts.
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