Compound Ranking
See on- and off-target activity across the proteome
Selectivity profiling helps researchers understand how compounds interact with their intended targets and identify potential off-target interactions. Selectivity can be assessed against predefined panels of proteins or through broader, proteome-wide approaches that capture responses beyond known targets. Profiling in native biological systems adds valuable biological context, supporting informed decisions about compound optimization and prioritization.
Understanding a compound’s selectivity is important for distinguishing desired target interactions from unintended effects that may influence efficacy, safety, or downstream biological responses. Comparing selectivity profiles across compounds can reveal differences in their interaction patterns, helping researchers identify promising candidates and guide further optimization.
Selectivity profiling is particularly valuable during lead optimization, where compounds are evaluated not only for potency against the intended target but also for their broader interaction profiles. Together with potency and other pharmacological data, selectivity information supports compound prioritization, guides structure–activity relationship (SAR) studies, and helps researchers select candidates for further development.
Look wider.
Decide earlier.
Compare compounds across the proteome without being limited to predefined target panels. CETSA Selectivity reveals shared, distinct, and unexpected thermal stability response patterns early enough to guide compound decisions
Selectivity Profiling is enabled by our CETSA Selectivity Service
CETSA Selectivity
Unbiased Selectivity Profiling with CETSA® and Mass Spectrometry
CETSA Selectivity enables unbiased analysis of compound selectivity in drug discovery—helping reduce off-target effects, improve safety, and accelerate informed decision-making.
What Is CETSA Selectivity?
While selectivity—the ability of a compound to modulate its primary target with minimal interaction with non-target proteins—has long been a pharmacological ideal, the methodologies used to evaluate it are undergoing a paradigm shift. At Pelago Bioscience, we advocate for a more integrative, data-driven approach: unbiased selectivity profiling with CETSA®.
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Unbiased Selectivity Profiling: with CETSA® and Mass Spectrometry
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CETSA: Your Key to Biological Relevance
Unlock Deeper Insights in Drug Discovery with CETSA Discover how to accelerate your drug discovery process with Pelago Bioscience’s exclusive eBook, CETSA: Your Key to Biological Relevance…