Unbiased Selectivity Profiling with CETSA® and Mass Spectrometry

Unbiased Selectivity Profiling: Revealing True Compound Specificity and Off-Target Interactions CETSA mass spectrometry profiling enables unbiased analysis of compound selectivity in drug discovery—helping reduce off-target effects, improve safety, and accelerate informed decision-making. This technique combines the power of CETSA® (Cellular Thermal Shift Assay) with quantitative mass spectrometry to identify how compounds interact with thousands of proteins in physiologically relevant systems. Pelago Bioscience’s Selectivity Profiling Service, powered by CETSA mass spectrometry profiling, offers a comprehensive and label-free approach to assess compound interactions across over 5,000 proteins in live cells or tissues. This innovative platform enables accurate identification of both on-tar...