Dissecting the Biology of Mutant p53 with CETSA®

Unlocking the Complex Biology of Mutant p53 with CETSA®: Revealing Target Engagement and Stability Profiles The tumor suppressor p53 is one of the most crucial proteins in preventing cancer, but its mutations often render it dysfunctional and a major challenge in drug development. Our study uses CETSA (Cellular Thermal Shift Assay) to investigate the binding and modulation of wild-type (WT) and mutant p53 across different breast cancer cell lines. By studying how these mutations alter p53’s thermal stability, we gain valuable insights into how tool compounds affect p53’s stability and function. Our work demonstrates how CETSA can be applied to dissect the distinct thermal stability profiles of both WT and mutant p53 in a cellular context, revealing key differences that could inform d...